Volumetric absorptive microsampling-LC-MS/MS assays for quantitation of giredestrant in dried human whole blood.
Ryan JohnsonImran RehmaniMark LeahyAbigail WalkerXiaorong LiangBrian DeanJianshuang WangPublished in: Bioanalysis (2023)
Volumetric absorption microsampling devices offer minimally invasive and user-friendly collection of capillary blood in volumes as low as 10 μl. Herein we describe the assay validation for determination of the selective estrogen receptor degrader giredestrant (GDC-9545) in dried human whole blood collected using the Mitra ® and Tasso-M20 devices. Both LC-MS/MS assays met validation acceptance criteria for the linear range 1-1000 ng/ml giredestrant. Mitra and Tasso-M20 samples were stable for 84 and 28 days at ambient conditions, respectively, and for 7-9 days at 40 and -70°C. Blood hematocrit, hyperlipidemia and anticoagulant did not impact quantitation of giredestrant. These validated assays are suitable for the determination of giredestrant in dried blood samples collected using Mitra and Tasso-M20 microsampling devices.
Keyphrases
- estrogen receptor
- high throughput
- endothelial cells
- solid phase extraction
- minimally invasive
- ms ms
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- induced pluripotent stem cells
- pluripotent stem cells
- molecularly imprinted
- high performance liquid chromatography
- air pollution
- venous thromboembolism
- tandem mass spectrometry
- high fat diet
- particulate matter
- metabolic syndrome
- tyrosine kinase
- high resolution
- skeletal muscle
- insulin resistance