A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos.
Shoichi NakamuraSeiji HiraMasato FujiwaraNasa MiyagataTakuma TsujiAkane KondoHitoshi KurumizakaYuko ShinozukaMakoto HayashiSatoru KobayashiMasanori MukaiPublished in: Communications biology (2019)
Expression of the vasa gene is associated with germline establishment. Therefore, identification of vasa activator(s) should provide insights into germline development. However, the genes sufficient for vasa activation remain unknown. Previously, we showed that the BTB/POZ-Zn-finger protein Mamo is necessary for vasa expression in Drosophila. Here, we show that the truncated Mamo lacking the BTB/POZ domain (MamoAF) is a potent vasa activator. Overexpression of MamoAF was sufficient to induce vasa expression in both primordial germ cells and brain. Indeed, Mamo mRNA encoding a truncated Mamo isoform, which is similar to MamoAF, was predominantly expressed in primordial germ cells. The results of our genetic and biochemical studies showed that MamoAF, together with CBP, epigenetically activates vasa expression. Furthermore, MamoAF and the germline transcriptional activator OvoB exhibited synergy in activating vasa transcription. We propose that a Mamo-mediated network of epigenetic and transcriptional regulators activates vasa expression.
Keyphrases
- transcription factor
- poor prognosis
- binding protein
- gene expression
- induced apoptosis
- genome wide
- dna methylation
- dna repair
- signaling pathway
- nuclear factor
- cell cycle arrest
- cell proliferation
- genome wide identification
- inflammatory response
- immune response
- dna binding
- dna damage
- subarachnoid hemorrhage
- toll like receptor
- heat shock
- long noncoding rna
- bioinformatics analysis
- germ cell
- heat shock protein
- embryonic stem cells