Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres.
Deborah FanfoneAhmed IdbaïhJade MammiMathieu GabutGabriel IchimPublished in: Cancers (2020)
Glioblastoma (GBM) is one of the cancers with the worst prognosis, despite huge efforts to understand its unusual heterogeneity and aggressiveness. This is mainly due to glioblastoma stem cells (GSCs), which are also responsible for the frequent tumor recurrence following surgery, chemotherapy or radiotherapy. In this study, we investigate the expression pattern of the anti-apoptotic BCL-xL protein in several GBM cell lines and the role it might play in GSC-enriched tumorspheres. We report that several GBM cell lines have an increased BCL-xL expression in tumorspheres compared to differentiated cells. Moreover, by artificially modulating BCL-xL expression, we unravel a correlation between BCL-xL and tumorsphere size. In addition, BCL-xL upregulation appears to sensitize GBM tumorspheres to newly developed BH3 mimetics, opening promising therapeutic perspectives for treating GBM patients.
Keyphrases
- poor prognosis
- stem cells
- cell death
- binding protein
- end stage renal disease
- long non coding rna
- induced apoptosis
- minimally invasive
- single cell
- signaling pathway
- ejection fraction
- cell proliferation
- prognostic factors
- squamous cell carcinoma
- bone marrow
- cell therapy
- radiation induced
- coronary artery bypass
- quality improvement
- patient reported outcomes
- endoplasmic reticulum stress
- amino acid
- pi k akt