Solid Lipid Nanoparticles Delivering a DNA Vaccine Encoding Helicobacter pylori Urease A Subunit: Immune Analyses before and after a Mouse Model of Infection.
Jasmine E FrancisIvana SkakicDebolina MajumdarAya C TakiRavi ShuklaAnna K WalduckPeter M SmookerPublished in: International journal of molecular sciences (2024)
In this study, novel solid lipid particles containing the adjuvant lipid monophosphoryl lipid A (termed 'SLN-A') were synthesised. The SLN-A particles were able to efficiently bind and form complexes with a DNA vaccine encoding the urease alpha subunit of Helicobacter pylori . The resultant nanoparticles were termed lipoplex-A. In a mouse model of H. pylori infection, the lipoplex-A nanoparticles were used to immunise mice, and the resultant immune responses were analysed. It was found that the lipoplex-A vaccine was able to induce high levels of antigen-specific antibodies and an influx of gastric CD4 + T cells in vaccinated mice. In particular, a prime with lipoplex-A and a boost with soluble UreA protein induced significantly high levels of the IgG1 antibody, whereas two doses of lipoplex-A induced high levels of the IgG2c antibody. In this study, lipoplex-A vaccination did not lead to a significant reduction in H. pylori colonisation in a challenge model; however, these results point to the utility of the system for delivering DNA vaccine-encoded antigens to induce immune responses and suggest the ability to tailor those responses.
Keyphrases
- helicobacter pylori
- mouse model
- immune response
- helicobacter pylori infection
- high glucose
- fatty acid
- circulating tumor
- early stage
- diabetic rats
- cell free
- high fat diet induced
- type diabetes
- squamous cell carcinoma
- oxidative stress
- radiation therapy
- skeletal muscle
- protein protein
- endothelial cells
- nucleic acid
- amino acid