Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements.
Dieter M TourlousseKoji NaritaTakamasa MiuraMitsuo SakamotoAkiko OhashiKeita ShiinaMasami MatsudaDaisuke MiuraMamiko ShimamuraYoshifumi OhyamaAtsushi YamazoeYoshihito UchinoKeishi KameyamaShingo AriokaJiro KataokaTakayoshi HisadaKazuyuki FujiiShunsuke TakahashiMiho KuroiwaMasatomo RokushimaMitsue NishiyamaYoshiki TanakaTakuya FuchikamiHitomi AokiSatoshi KiraRyo KoyanagiTakeshi NaitoMorie NishiwakiHirotaka KumagaiMikiko KondaKen KasaharaMoriya OhkumaHiroko KawasakiYuji SekiguchiJun TerauchiPublished in: Microbiome (2021)
The validated protocols and methodological guidance for DNA extraction and library construction provided in this study expand current best practices for metagenomic analyses of human fecal microbiota. Uptake of our protocols and guidelines will improve the accuracy and comparability of metagenomics-based studies of the human microbiome, thereby facilitating development and commercialization of human microbiome-based products. Video Abstract.