Long-Term Effects of Suramin on Renal Function in Streptozotocin-Induced Diabetes in Rats.
Gabriela ChyłaKornelia Sałaga-ZaleskaEwelina KreftOlaf StumskiAleksandra KrzesińskaMonika Sakowicz-BurkiewiczAgnieszka KuchtaMaciej JankowskiPublished in: International journal of molecular sciences (2023)
In short-term diabetes (3 weeks), suramin, a drug used clinically, affects renal function and the expression of vascular endothelial growth factor A (VEGF-A), which may be involved in the pathogenesis of diabetic nephropathy, the main cause of end-stage renal disease. In the present study, we evaluated the long-term (11 weeks) effects of suramin (10 mg/kg, i.p. , once-weekly) in diabetic rats. Concentrations of VEGF-A, albumin, soluble adhesive molecules (sICAM-1, sVCAM-1), nucleosomes, and thrombin-antithrombin complex (TAT) were measured by ELISA, total protein was measured using a biuret reagent. Glomerular expression of VEGF-A was evaluated by Western blot, mRNA for VEGF-A receptors in the renal cortex by RT-PCR. The vasoreactivity of the interlobar arteries to acetylcholine was assessed by wire myography. Long-term diabetes led to an increased concentration of VEGF-A, TAT, and urinary excretion of total protein and albumin, and a decrease in the concentration of sVCAM-1. We have shown that suramin in diabetes reduces total urinary protein excretion and restores the relaxing properties of acetylcholine relaxation properties to non-diabetic levels. Suramin had no effect on glomerular expression VEGF-A expression and specific receptors, and on sICAM-1 and nucleosomes concentrations in diabetic rats. In conclusion, the long-term effect of suramin on the kidneys in diabetes, expressed in the reduction of proteinuria and the restoration of endothelium-dependent relaxation of the renal arteries, can be considered as potentially contributing to the reduction/slowing down of the development of diabetic nephropathy.
Keyphrases
- vascular endothelial growth factor
- diabetic rats
- diabetic nephropathy
- type diabetes
- endothelial cells
- oxidative stress
- poor prognosis
- binding protein
- cardiovascular disease
- glycemic control
- high glucose
- end stage renal disease
- chronic kidney disease
- peritoneal dialysis
- insulin resistance
- protein protein
- high fat diet
- amino acid
- functional connectivity
- nitric oxide
- long non coding rna
- drug induced