Sphingomicrobium clamense sp. nov., Isolated from Sediment of Clam Island Beach in China.

A Gram-stain-negative, non-flagellated, aerobic, ovoid or rod-shaped bacterium with motility, designated B8 T , was isolated from the sediment of Clam Island beach, Liaoning province, China. The optimum growth of strain B8 T occurred at 35 o C, pH 7.0, and in the presence of 4.0-5.0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B8 T formed a distinct lineage within the genus Sphingomicrobium and was closely related to Sphingomicrobium nitratireducens O-35 T (98.3% sequence similarity), Sphingomicrobium aestuariivivum KCTC 42286 T (96.9%), and Sphingomicrobium astaxanthinifaciens JCM 18551 T (96.5%). The digital DNA-DNA hybridization and average nucleotide identity values between strain B8 T and closely related strains were lower than 21.0% and 78.0%, much lower than the cutoff values of 70.0% and 95.0%, respectively, for bacterial species delineation. The dominant respiratory quinone of strain B8 T was ubiquinone-10. The major fatty acids were Sum In Feature 8 (C 18:1 ω7c and/or C 18:1 ω6c), Sum In Feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c), C 17:1 ω6c, C 18:1 2-OH, and C 16:0 . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, glycolipids, and four unknown polar lipids. The DNA G + C content of strain B8 T was 63.9%. Based on the phenotypic, phylogenetic, and chemotaxonomic analyses, strain B8 T is considered a new species of Sphingomicrobium, for which the name Sphingomicrobium clamense sp. nov. is proposed. The type strain is B8 T (= CGMCC 1.19486 T  = KCTC 92052 T ).