MALDI TIMS IMS Reveals Ganglioside Molecular Diversity within Murine S. aureus Kidney Tissue Abscesses.
Katerina V DjambazovaKatherine N Gibson-CorleyJeffrey A FreibergRichard Micheal CaprioliEric P SkaarJeffrey M SpragginsPublished in: Journal of the American Society for Mass Spectrometry (2024)
Gangliosides play important roles in innate and adaptive immunity. The high degree of structural heterogeneity results in significant variability in ganglioside expression patterns and greatly complicates linking structure and function. Structural characterization at the site of infection is essential in elucidating host ganglioside function in response to invading pathogens, such as Staphylococcus aureus ( S. aureus ). Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables high-specificity spatial investigation of intact gangliosides. Here, ganglioside structural and spatial heterogeneity within an S. aureus -infected mouse kidney abscess was characterized. Differences in spatial distributions were observed for gangliosides of different classes and those that differ in ceramide chain composition and oligosaccharide-bound sialic acid. Furthermore, integrating trapped ion mobility spectrometry (TIMS) allowed for the gas-phase separation and visualization of monosialylated ganglioside isomers that differ in sialic acid type and position. The isomers differ in spatial distributions within the host-pathogen interface, where molecular patterns revealed new molecular zones in the abscess previously unidentified by traditional histology.
Keyphrases
- mass spectrometry
- high resolution
- staphylococcus aureus
- single cell
- immune response
- liquid chromatography
- gas chromatography
- single molecule
- poor prognosis
- capillary electrophoresis
- high performance liquid chromatography
- escherichia coli
- rare case
- room temperature
- cystic fibrosis
- multidrug resistant
- long non coding rna
- methicillin resistant staphylococcus aureus
- ms ms
- tandem mass spectrometry