Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis.
Hongyuan ZhaoHaoqiang ZhaoYuanfeng HuShanshan ZhangShao-Zhen HeHuan ZhangNing ZhaoQingchang LiuShao-Pei GaoHong ZhaiPublished in: Genes (2022)
Sweet potato ( Ipomoea batatas (L.) Lam) is one of the most crucial food crops widely cultivated worldwide. In plants, MYB transcription factors play crucial roles in plant growth, defense regulation, and stress resistance. However, the regulatory mechanism of MYBs in salt and drought response remain poorly studied in sweet potato. By screening a transcriptome database for differentially expressed genes between the sweet potato variety Jingshu 6 and its mutant JS6-5 with high anthocyanin and increased tolerance to salt and drought stresses, we identified a R2R3-MYB gene IbMYB48 , for which expression was induced by PEG6000, NaCl, abscisic acid (ABA), methyl jasmonic acid (MeJA), salicylic acid (SA) and H 2 O 2 . Particle-mediated transient transformation of onion epidermal cells showed IbMYB48 is localized in the nucleus. Transactivation activity assay in yeast cells revealed that IbMYB48 has transactivation activity, and its active domain is located in the carboxyl (C)-terminal region. Furthermore, expression of IbMYB48 confers enhanced tolerance to salt and drought stresses in transgenic Arabidopsis. The contents of endogenous ABA, JA, and proline in transgenic lines were higher than control, and the activity of superoxide dismutase (SOD) was significantly increased under salt and drought stress conditions. By contrast, the accumulation of malondialdehyde (MDA) and H 2 O 2 were lower. Moreover, genes encoding enzymes involved in ABA biosynthetic pathway, JA biosynthesis and signaling pathway, and reactive oxygen species (ROS) scavenging system were significantly up-regulated in transgenic Arabidopsis under salt or drought stress. Altogether, these results suggest IbMYB48 may be a candidate gene for improvement of abiotic stress tolerance.
Keyphrases
- transcription factor
- genome wide identification
- plant growth
- induced apoptosis
- poor prognosis
- dna binding
- genome wide
- arabidopsis thaliana
- climate change
- signaling pathway
- reactive oxygen species
- cell cycle arrest
- heat stress
- magnetic resonance
- binding protein
- oxidative stress
- pi k akt
- long non coding rna
- single cell
- emergency department
- epithelial mesenchymal transition
- hydrogen peroxide
- copy number
- brain injury
- computed tomography
- human health
- subarachnoid hemorrhage
- amyotrophic lateral sclerosis
- breast cancer cells
- rna seq
- nitric oxide
- genome wide analysis