Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195.
Ming-Shian LeeShih-Wei WangGuei-Jane WangKa-Lai PangChing-Kuo LeeYueh-Hsiung KuoHyo-Jung ChaRuo-Kai LinTzong-Huei LeePublished in: Journal of natural products (2016)
Seven new polyketides, phomaketides A-E (1-5) and pseurotins A3 (6) and G (7), along with the known compounds FR-111142, pseurotins A, A1, A2, D, and F2, 14-norpseurotin A, α-carbonylcarbene, tyrosol, cyclo(-l-Pro-l-Leu), and cyclo(-l-Pro-l-Phe), were purified from the fermentation broth and mycelium of the endophytic fungal strain Phoma sp. NTOU4195 isolated from the marine red alga Pterocladiella capillacea. The structures were established through interpretation of spectroscopic data. The antiangiogenic and anti-inflammatory effects of 1-7 and related analogues were evaluated using human endothelial progenitor cells (EPCs) and lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, respectively. Of the compounds tested, compound 1 exhibited the most potent antiangiogenic activity by suppressing the tube formation of EPCs with an IC50 of 8.1 μM, and compound 3 showed the most selective inhibitory activity of LPS-induced NO production in RAW264.7 macrophages with an IC50 value of 8.8 μM.
Keyphrases
- anti inflammatory
- lps induced
- endothelial cells
- inflammatory response
- molecular docking
- induced apoptosis
- cell cycle arrest
- vascular endothelial growth factor
- electronic health record
- adipose tissue
- high resolution
- toll like receptor
- induced pluripotent stem cells
- endoplasmic reticulum stress
- big data
- pluripotent stem cells
- molecular dynamics simulations
- saccharomyces cerevisiae
- cell death
- oxidative stress
- machine learning
- cell proliferation
- wound healing
- data analysis
- mass spectrometry
- structure activity relationship