8-Oxoguanine DNA Glycosylase 1 Upregulation as a Risk Factor for Obesity and Colorectal Cancer.
Jesús PiloLibia Alejandra García-FloresMercedes Clemente-PostigoIsabel Arranz-SalasJulia AlcaideMaria Ramos-FernandezJosé LozanoHatim BoughanemPallavi KompellaManuel Macias-GonzálezPublished in: International journal of molecular sciences (2023)
DNA damage has been extensively studied as a potentially helpful tool in assessing and preventing cancer, having been widely associated with the deregulation of DNA damage repair (DDR) genes and with an increased risk of cancer. Adipose tissue and tumoral cells engage in a reciprocal interaction to establish an inflammatory microenvironment that enhances cancer growth by modifying epigenetic and gene expression patterns. Here, we hypothesize that 8-oxoguanine DNA glycosylase 1 (OGG1)-a DNA repair enzyme-may represent an attractive target that connects colorectal cancer (CRC) and obesity. In order to understand the mechanisms underlying the development of CRC and obesity, the expression and methylation of DDR genes were analyzed in visceral adipose tissue from CRC and healthy participants. Gene expression analysis revealed an upregulation of OGG1 expression in CRC participants ( p < 0.005) and a downregulation of OGG1 in normal-weight healthy patients ( p < 0.05). Interestingly, the methylation analysis showed the hypermethylation of OGG1 in CRC patients ( p < 0.05). Moreover, expression patterns of OGG1 were found to be regulated by vitamin D and inflammatory genes. In general, our results showed evidence that OGG1 can regulate CRC risk through obesity and may act as a biomarker for CRC.
Keyphrases
- dna repair
- dna damage
- insulin resistance
- adipose tissue
- poor prognosis
- gene expression
- genome wide
- weight loss
- metabolic syndrome
- oxidative stress
- end stage renal disease
- dna damage response
- dna methylation
- type diabetes
- weight gain
- genome wide identification
- papillary thyroid
- ejection fraction
- newly diagnosed
- long non coding rna
- squamous cell
- high fat diet induced
- chronic kidney disease
- high fat diet
- peritoneal dialysis
- cell proliferation
- prognostic factors
- stem cells
- body mass index
- induced apoptosis
- signaling pathway
- cell free
- transcription factor
- copy number
- multidrug resistant
- lymph node metastasis
- squamous cell carcinoma
- circulating tumor cells
- cell death