Engineering probiotics as living diagnostics and therapeutics for improving human health.
Zhao ZhouXin ChenHuakang ShengXiaolin ShenXinxiao SunYajun YanJia WangQipeng YuanPublished in: Microbial cell factories (2020)
The gut microbiota that inhabit our gastrointestinal tract are well known to play an important role in maintaining human health in many aspects, including facilitating the digestion and absorption of nutrients, protecting against pathogens and regulating immune system. Gut microbiota dysbiosis is associated with a lot of diseases, such as inflammatory bowel disease, allergy, obesity, cardiovascular and neurodegenerative diseases and cancers. With the increasing knowledge of the microbiome, utilization of probiotic bacteria in modulating gut microbiota to prevent and treat a large number of disorders and diseases has gained much interest. In recent years, aided by the continuous development of tools and techniques, engineering probiotic microbes with desired characteristics and functionalities to benefit human health has made significant progress. In this paper, we summarize the recent advances in design and construction of probiotics as living diagnostics and therapeutics for probing and treating a series of diseases including metabolic disorders, inflammation and pathogenic bacteria infections. We also discuss the current challenges and future perspectives in expanding the application of probiotics for disease treatment and detection. We intend to provide insights and ideas for engineering of probiotics to better serve disease therapy and human health.
Keyphrases
- human health
- risk assessment
- climate change
- heavy metals
- small molecule
- metabolic syndrome
- healthcare
- oxidative stress
- insulin resistance
- type diabetes
- weight loss
- bacillus subtilis
- signaling pathway
- stem cells
- gram negative
- adipose tissue
- skeletal muscle
- bone marrow
- multidrug resistant
- weight gain
- molecular dynamics simulations
- physical activity
- replacement therapy
- lactic acid
- infectious diseases
- sensitive detection