An Integrative Systems Biology Approach Identifies Molecular Signatures Associated with Gallbladder Cancer Pathogenesis.
Nabanita RoyMrinmoy KshattrySusmita MandalMohit Kumar JollyDhruba Kumar BhattacharyyaPankaj BarahPublished in: Journal of clinical medicine (2021)
Gallbladder cancer (GBC) has a lower incidence rate among the population relative to other cancer types but is a major contributor to the total number of biliary tract system cancer cases. GBC is distinguished from other malignancies by its high mortality, marked geographical variation and poor prognosis. To date no systemic targeted therapy is available for GBC. The main objective of this study is to determine the molecular signatures correlated with GBC development using integrative systems level approaches. We performed analysis of publicly available transcriptomic data to identify differentially regulated genes and pathways. Differential co-expression network analysis and transcriptional regulatory network analysis was performed to identify hub genes and hub transcription factors (TFs) associated with GBC pathogenesis and progression. Subsequently, we assessed the epithelial-mesenchymal transition (EMT) status of the hub genes using a combination of three scoring methods. The identified hub genes including, CDC6, MAPK15, CCNB2, BIRC7, L3MBTL1 were found to be regulators of cell cycle components which suggested their potential role in GBC pathogenesis and progression.
Keyphrases
- network analysis
- poor prognosis
- papillary thyroid
- genome wide
- cell cycle
- transcription factor
- bioinformatics analysis
- epithelial mesenchymal transition
- squamous cell
- genome wide identification
- gene expression
- squamous cell carcinoma
- lymph node metastasis
- type diabetes
- risk factors
- signaling pathway
- dna methylation
- binding protein
- drug induced