Oleic acid differentially affects lipid droplet storage of de novo synthesized lipids in hepatocytes and adipocytes.
Hannah B CastilloSydney O ShusterLydia H TarekegnCaitlin M DavisPublished in: Chemical communications (Cambridge, England) (2024)
Lipogenesis is a vital but often dysregulated metabolic pathway. Here we use optical photothermal infrared imaging to quantify lipogenesis rates of isotopically labelled oleic acid and glucose concomitantly in live cells. In hepatocytes, but not adipocytes, we find that oleic acid feeding at 60 μM increases the number and size of lipid droplets (LDs) while simultaneously inhibiting storage of de novo synthesized lipids in LDs. Our results demonstrate alternate regulation of lipogenesis between cell types.
Keyphrases
- high fat diet induced
- fatty acid
- high resolution
- adipose tissue
- single cell
- induced apoptosis
- signaling pathway
- drug delivery
- insulin resistance
- photodynamic therapy
- cell therapy
- liver injury
- type diabetes
- blood glucose
- metabolic syndrome
- skeletal muscle
- cell cycle arrest
- endoplasmic reticulum stress
- high speed
- cell proliferation
- pi k akt