Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology.
Yujie LiuGuanliu YuHongkun LiangWenbo SunLulu ZhangMichael G MaukHua LiLei ChenPublished in: Analytical methods : advancing methods and applications (2024)
As of now, the global COVID-19 pandemic caused by SARS-CoV-2, which began in 2019, has been effectively controlled. However, the symptoms of influenza A virus infection were similar to those of SARS-CoV-2 infection, but they required different treatment approaches. To make the detection more accurate and the treatment more targeted. We developed a system that integrates RPA and CRISPR assays, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2. Under isothermal amplification conditions, the RPA-CRISPR Cas12a detection system achieved a detection limit as low as 5 copies per μL, demonstrating excellent specificity. The measurement time was approximately 30 minutes. The RPA-CRISPR Cas12a detection system combined with the microfluidic chip we designed to simultaneously detect three viruses, providing a potential solution for efficient and reliable diagnosis.
Keyphrases
- loop mediated isothermal amplification
- sars cov
- crispr cas
- label free
- sensitive detection
- genome editing
- real time pcr
- high throughput
- respiratory syndrome coronavirus
- circulating tumor cells
- genome wide
- gene expression
- high resolution
- dna methylation
- risk assessment
- cancer therapy
- mass spectrometry
- combination therapy