Large Extracellular Vesicles Can be Characterised by Multiplex Labelling Using Imaging Flow Cytometry.

Suzanne M JohnsonAntonia BanyardChristopher SmithAleksandr MironovMartin G McCabe

Published in: International journal of molecular sciences (2020)

We have developed a labelling and stringent gating strategy which is able to explore EV marker expression (CD63, CD9, and LAMP1) on individual EVs within a widely heterogeneous population. Taken together, data presented here strongly support the value of exploring large EVs in clinical samples for potential biomarkers, useful in diagnostic screening and disease monitoring.

Keyphrases

flow cytometry
poor prognosis
high resolution
electronic health record
high throughput
big data
binding protein
loop mediated isothermal amplification
real time pcr
mass spectrometry
machine learning
long non coding rna
data analysis
sensitive detection