Tailoring structural properties of spray-dried methotrexate-loaded poly (lactic acid)/poloxamer microparticle blends.
Edilene Gadelha de OliveiraPaula Renata Lima MachadoKleber Juvenal Silva FariasTiago R da CostaDulce Maria Araújo MeloAriane Ferreira LacerdaMatheus de Freitas Fernandes-PedrosaAlianda Maira CornélioArnóbio Antônio da Silva JúniorPublished in: Journal of materials science. Materials in medicine (2019)
Drug delivery systems can overcome cancer drug resistance, improving the efficacy of chemotherapy agents. Poly (lactic acid) (PLA) microparticles are an interesting alternative because their hydrophobic surface and small particle size could facilitate interactions with cells. In this study, two poloxamers (PLX 407 and 188) were applied to modulate the structural features, the drug release behavior and the cell viability from spray-dried microparticles. Five formulations with different PLA: PLX blend ratio (100:0, 75:25, 50:50, 25:50, and 0:100) were well-characterized by SEM, particle size analysis, FTIR spectroscopy, differential scanning calorimetry (DSC), and X-ray diffraction analysis (XRD). The spray-dried microparticles showed higher drug loading, spherical-shape, and smaller particle size. The type of poloxamer and blend ratio affected their structural and functional properties such as morphology, crystallinity, blend miscibility, drug release rate, and cell viability. The methotrexate (MTX), a model drug, was loaded in amorphous spray-dried microparticles. Moreover, the drug release studies demonstrated that PLX induced a leaching-effect of MTX from PLA: PLX blends, suggesting the formation of MTX/PLX micelles in aqueous medium. This finding was better established by cell viability assays. Therefore, biocompatible PLA: PLX blends showed promising in vitro results, and further in vivo studies will be performed to evaluate the performance of this chemotherapeutic agent.
Keyphrases
- drug release
- drug delivery
- lactic acid
- high resolution
- cancer therapy
- induced apoptosis
- electron microscopy
- drug induced
- wound healing
- ionic liquid
- computed tomography
- cell proliferation
- radiation therapy
- papillary thyroid
- single molecule
- magnetic resonance imaging
- high throughput
- mass spectrometry
- locally advanced
- squamous cell carcinoma
- oxidative stress
- adverse drug
- signaling pathway
- data analysis