Metabolic phenotyping of healthy and diseased human RPE cells.

Saira RizwanBeverly ToothmanBo LiAbbi J EngelRayne R LimSheldon NiernbergerJinyu LuCloe RatliffYinxiao XiangMark EminhizerJennifer R ChaoJianhai Du

Published in: bioRxiv : the preprint server for biology (2024)

Epithelial phenotype confers metabolic flexibility to healthy RPE for utilizing various nutrients. SFD RPE cells have reduced metabolic flexibility, relying on the oxidation of BCAAs. Our findings highlight the potentially important roles of nutrient availability and utilization in RPE differentiation and diseases.

Keyphrases

induced apoptosis
cell cycle arrest
endothelial cells
endoplasmic reticulum stress
high throughput
nitric oxide
hydrogen peroxide
heavy metals
cell proliferation
pluripotent stem cells