Zyxin as a potential cancer prognostic marker promotes the proliferation and metastasis of colorectal cancer cells.
Chenhan ZhongJiekai YuDan LiKai JiangYang TangMengyuan YangHong ShenXuefeng FangKe-Feng DingShu ZhengYing YuanPublished in: Journal of cellular physiology (2019)
Colorectal cancer (CRC) is a leading cause of cancer death. This study was conducted to investigate the functions and mechanisms of Zyxin (ZYX) in CRC. Multiomics analysis associated ZYX with CRC metastasis. ZYX expression levels were increased in human CRC tissues and related to shorter recurrence-free survival. Knockdown of ZYX expression resulted in inhibition of cell growth, invasion, and migration in vitro and in vivo. Comprehensive analysis of gene microarray analysis showed that ZYX may activate the pathway of NUPR1 and JNK, inhibit CST5, regulate focal adhesion (FA), and affect epithelial-mesenchymal transition in CRC cells. Results of gene microarray and membrane protein isobaric tags with relative and absolute quantitation labeling mass spectrometry found ten differentially expressed genes, which were associated with ZYX activity. Furthermore, real-time polymerase chain reaction was used to validate the expression patterns of selected genes in the integrative analysis. Taken together, our findings provide the first evidence that decreased expression level of ZYX impairs CRC cell proliferation and metastasis probably via the FA pathway.
Keyphrases
- poor prognosis
- mass spectrometry
- cell proliferation
- free survival
- epithelial mesenchymal transition
- genome wide
- signaling pathway
- papillary thyroid
- endothelial cells
- genome wide identification
- copy number
- long non coding rna
- liquid chromatography
- young adults
- bioinformatics analysis
- squamous cell
- staphylococcus aureus
- transcription factor
- endoplasmic reticulum stress
- dna methylation
- tandem mass spectrometry
- high performance liquid chromatography
- pi k akt
- cell cycle arrest
- simultaneous determination
- human health