Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Tal GordonTal ZaquinMark Alec KowarskyYotam VoskoboynikNoam HendinOmri WurtzelFederico CaicciLucia ManniAyelet VoskoboynikNoa ShenkarPublished in: Cells (2022)
Understanding how neurons regenerate following injury remains a central challenge in regenerative medicine. Adult mammals have a very limited ability to regenerate new neurons in the central nervous system (CNS). In contrast, the basal chordate Polycarpa mytiligera can regenerate its entire CNS within seven days of complete removal. Transcriptome sequencing, cellular labeling, and proliferation in vivo essays revealed that CNS regeneration is mediated by a newly formed neural progeny and the activation of neurodevelopmental pathways that are associated with enhanced stem-cell activity. Analyzing the expression of 239 activated pathways enabled a quantitative understanding of gene-set enrichment patterns at key regeneration stages. The molecular and cellular mechanisms controlling the regenerative ability that this study reveals can be used to develop innovative approaches to enhancing neurogenesis in closely-related chordate species, including humans.
Keyphrases
- stem cells
- single cell
- blood brain barrier
- spinal cord
- genome wide
- cerebral ischemia
- poor prognosis
- cell therapy
- rna seq
- gene expression
- magnetic resonance
- signaling pathway
- high resolution
- copy number
- white matter
- brain injury
- computed tomography
- spinal cord injury
- single molecule
- young adults
- magnetic resonance imaging
- neural stem cells
- transcription factor
- mesenchymal stem cells
- cancer stem cells