Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
Eduarda R OliveiraLeonara FayerRafaella S S ZanetteLuiz O LadeiraLuiz F C de OliveiraCarlos M C MarandubaHumberto M BrandãoMichele MunkPublished in: Nanotechnology (2021)
Carboxylated multi-wall carbon nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNT-COOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields.
Keyphrases
- carbon nanotubes
- stem cells
- endothelial cells
- tissue engineering
- induced pluripotent stem cells
- pluripotent stem cells
- cell therapy
- induced apoptosis
- mesenchymal stem cells
- hydrogen peroxide
- risk assessment
- single cell
- reactive oxygen species
- climate change
- cell proliferation
- cell free
- mass spectrometry
- atomic force microscopy
- postmenopausal women
- heat stress
- human health
- pi k akt