Dynamics of epigenetic modifications in ICSI embryos from in vitro-produced spermatozoa.

Antoine ObletteAurélie Rives-FerailleLudovic DumontMarion DelessardJustine SaulnierNathalie Rives Christine Rondanino

Published in: Andrology (2020)

Our study shows for the first time that the use of in vitro-produced spermatozoa alters DNA methylation/demethylation dynamics but has little impact on H3K4me3, H3K27me3 and H3K9ac levels in mouse early embryos. Further work will have to be performed to determine whether the use of these gametes is not deleterious for embryo development before considering a human application.

Keyphrases

dna methylation
gene expression
endothelial cells
genome wide
pregnancy outcomes
induced pluripotent stem cells
pluripotent stem cells
pregnant women