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Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter.

Shahana BegumMohammed NooruzzamanAzmary HasnatMst Murshida ParvinRokshana ParvinMohammed Rafiqul IslamEmdadul Hauqe Chowdhury
Published in: Veterinary medicine and science (2020)
The findings suggest that primary GK cells can be successfully prepared from the mature kidney cortical tissues and can be used for the isolation of PPRV. This system could reduce the unnecessary sacrifice of lambs or kids. Since kidneys of slaughtered goats are available throughout the year, using this protocol primary cell culture from mature goat kidney can provide primary cells to the laboratory throughout the year.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • randomized controlled trial
  • gene expression
  • endoplasmic reticulum stress
  • cell death
  • signaling pathway