Genomic DNA Extracted from Lactiplantibacillus plantarum Attenuates Porphyromonas gingivalis Lipopolysaccharide (LPS)-Induced Inflammatory Responses via Suppression of Toll-Like Receptor (TLR)-Mediated Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor-κB (NF-κB) Signaling Pathways.
Young Hyeon ChoiBong Sun KimSeok-Seong KangPublished in: Food science of animal resources (2023)
In the present study, we aimed to examine the inhibition of genomic DNA from Lactiplantibacillus plantarum (LpDNA) on Porphyromonas gingivalis lipopolysaccharide (PgLPS)-induced inflammatory responses in RAW264.7 cells. Pretreatment with LpDNA for 15 h significantly inhibited PgLPS-induced mRNA expression and protein secretion of interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein-1. LpDNA pretreatment also reduced the mRNA expression of Toll-like receptor (TLR)2 and TLR4. Furthermore, LpDNA inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) and the activation of nuclear factor-κB (NF-κB) induced by PgLPS. Taken together, these findings demonstrate that LpDNA attenuates PgLPS-induced inflammatory responses by regulating MAPKs and NF-κB signaling pathways through the suppression of TLR2 and TLR4 expression.
Keyphrases
- toll like receptor
- nuclear factor
- inflammatory response
- lps induced
- signaling pathway
- immune response
- high glucose
- diabetic rats
- induced apoptosis
- pi k akt
- oxidative stress
- circulating tumor
- endothelial cells
- drug induced
- cell free
- poor prognosis
- dendritic cells
- single molecule
- protein kinase
- epithelial mesenchymal transition
- copy number
- cell death
- tyrosine kinase
- nucleic acid
- small molecule
- long non coding rna
- peripheral blood