Absolute quantitation of microbiota abundance in environmental samples.
Andrzej TkaczMarion HortalaPhilip Simon PoolePublished in: Microbiome (2018)
Spikes can be adapted to any amplicon-specific group including rhizobia from soils, Firmicutes and Bifidobacteria from human gut or Enterobacteriaceae from food samples. Crucially, using highly complex soil samples, we show that the absolute abundance of specific groups can remain steady or increase, even when their relative abundance decreases. Thus, without absolute quantitation, the underlying pathology, physiology and ecology of microbial groups may be masked by their relative abundance.
Keyphrases
- antibiotic resistance genes
- microbial community
- ms ms
- human health
- mass spectrometry
- endothelial cells
- liquid chromatography tandem mass spectrometry
- wastewater treatment
- pseudomonas aeruginosa
- multidrug resistant
- risk assessment
- tandem mass spectrometry
- escherichia coli
- klebsiella pneumoniae
- pluripotent stem cells