Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.
Nurlan SandybayevVyacheslav BeloussovVitaliy StrochkovMaxim SolomadinJoanna GranicaSergey YegorovPublished in: Microorganisms (2022)
The COVID-19 pandemic and heightened perception of the risk of emerging viral infections have boosted the efforts to better understand the virome or complete repertoire of viruses in health and disease, with a focus on infectious respiratory diseases. Next-generation sequencing (NGS) is widely used to study microorganisms, allowing the elucidation of bacteria and viruses inhabiting different body systems and identifying new pathogens. However, NGS studies suffer from a lack of standardization, in particular, due to various methodological approaches and no single format for processing the results. Here, we review the main methodological approaches and key stages for studies of the human virome, with an emphasis on virome changes during acute respiratory viral infection, with applications for clinical diagnostics and epidemiologic analyses.
Keyphrases
- respiratory tract
- copy number
- endothelial cells
- healthcare
- public health
- case control
- mental health
- circulating tumor
- respiratory failure
- health information
- dna methylation
- drug induced
- social media
- antimicrobial resistance
- genetic diversity
- genome wide
- induced pluripotent stem cells
- pluripotent stem cells
- extracorporeal membrane oxygenation
- high throughput sequencing
- cell free