Promoter Variation and Gene Expression of mcr-1-Harboring Plasmids in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese Hospital.
Bin HuangYuting HeXingyan MaRenxin CaiJianming ZengYang LuWeizheng ZhangKai LanSunmei EYi-Wei TangBarry N KreiswirthCha ChenLiang ChenPublished in: Antimicrobial agents and chemotherapy (2018)
Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- gene expression
- dna methylation
- multidrug resistant
- copy number
- healthcare
- genome wide
- transcription factor
- poor prognosis
- biofilm formation
- drug resistant
- gram negative
- amino acid
- optical coherence tomography
- emergency department
- pseudomonas aeruginosa
- binding protein
- acute care