Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016.
Adriana LuchsKaelan TardyRoozbeh TahmasebiSimone Guadagnucci MorilloFlavio Augusto de Pádua MilagresVanessa Dos Santos MoraisRafael BrustulinMaria da Aparecida Rodrigues TelesLais Sampaio de AzevedoEllen Viana de SouzaRoberta Salzone MedeirosYasmin França Viana Pires de SouzaEmerson Luiz Lima AraújoSteven S WitkinXutao DengEric DelwartEster Cerdeira SabinoElcio LealAntonio Charlys da CostaPublished in: Archives of virology (2021)
This study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.