Forkhead box family transcription factors as versatile regulators for cellular reprogramming to pluripotency.
Meijun FuHuan ChenZepo CaiYihang YangZiyu FengMengying ZengLijun ChenYue QinBaomei CaiPinghui ZhuChunhua ZhouShengyong YuJing GuoJing LiuShangtao CaoDuanqing PeiPublished in: Cell regeneration (London, England) (2021)
Forkhead box (Fox) transcription factors play important roles in mammalian development and disease. However, their function in mouse somatic cell reprogramming remains unclear. Here, we report that FoxD subfamily and FoxG1 accelerate induced pluripotent stem cells (iPSCs) generation from mouse fibroblasts as early as day4 while FoxA and FoxO subfamily impede this process obviously. More importantly, FoxD3, FoxD4 and FoxG1 can replace Oct4 respectively and generate iPSCs with germline transmission together with Sox2 and Klf4. On the contrary, FoxO6 almost totally blocks reprogramming through inhibiting cell proliferation, suppressing the expression of pluripotent genes and hindering the process of mesenchymal to epithelial transition (MET). Thus, our study uncovers unexpected roles of Fox transcription factors in reprogramming and offers new insights into cell fate transition.
Keyphrases
- transcription factor
- genome wide identification
- induced pluripotent stem cells
- cell fate
- cell proliferation
- dna binding
- signaling pathway
- poor prognosis
- stem cells
- single cell
- pi k akt
- genome wide
- cell cycle
- optical coherence tomography
- tyrosine kinase
- oxidative stress
- diabetic retinopathy
- dna damage
- dna methylation
- optic nerve