Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers.
Huijing LinChang-Lin HsiehKeng-Chia HsuPei-Hsuan LiaoSufang QiuTianxun GongKen-Tye YongShangyuan FengKien Voon KongPublished in: Nature communications (2021)
The limited availability of nasopharyngeal carcinoma-related progression biomarker array kits that offer physicians comprehensive information is disadvantageous for monitoring cancer progression. To develop a biomarker array kit, systematic identification and differentiation of a large number of distinct molecular surface-enhanced Raman scattering (SERS) reporters with high spectral temporal resolution is a major challenge. To address this unmet need, we use the chemistry of metal carbonyls to construct a series of unique SERS reporters with the potential to provide logical and highly multiplex information during testing. In this study, we report that geometric control over metal carbonyls on nanotags can produce 14 distinct barcodes that can be decoded unambiguously using commercial Raman spectroscopy. These metal carbonyl nanobarcodes are tested on human blood samples and show strong sensitivity (0.07 ng/mL limit of detection, average CV of 6.1% and >92% degree of recovery) and multiplexing capabilities for MMPs.
Keyphrases
- raman spectroscopy
- label free
- real time pcr
- gold nanoparticles
- sensitive detection
- high throughput
- endothelial cells
- loop mediated isothermal amplification
- primary care
- high resolution
- papillary thyroid
- single molecule
- health information
- magnetic resonance imaging
- high density
- computed tomography
- risk assessment
- optical coherence tomography
- human health
- social media
- pluripotent stem cells
- quantum dots
- bioinformatics analysis
- transition metal