(E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) Phenol Ameliorates MPTP-Induced Dopaminergic Neurodegeneration by Inhibiting the STAT3 Pathway.
Ji Yeon ChoiJaesuk YunChul Ju HwangHee Pom LeeHae Deun KimHyungok ChunPil-Hoon ParkDong Young ChoiSang-Bae HanJin Tae HongPublished in: International journal of molecular sciences (2019)
Neuroinflammation is implicated in dopaminergic neurodegeneration. We have previously demonstrated that (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol (MMPP), a selective signal transducer and activator of transcription 3 (STAT3) inhibitor, has anti-inflammatory properties in several inflammatory disease models. We investigated whether MMPP could protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic cell loss and behavioral impairment. Imprinting control region (ICR) mice (8 weeks old, n = 10 per group) were administered MMPP (5 mg/kg) in drinking water for 1 month, and injected with MPTP (15 mg/kg, four times with 2 h intervals) during the last 7 days of treatment. MMPP decreased MPTP-induced behavioral impairments in rotarod, pole, and gait tests. We also showed that MMPP ameliorated dopamine depletion in the striatum and inflammatory marker elevation in primary cultured neurons by high-performance liquid chromatography and immunohistochemical analysis. Increased activation of STAT3, p38, and monoamine oxidase B (MAO-B) were observed in the substantia nigra and striatum after MPTP injection, effects that were attenuated by MMPP treatment. Furthermore, MMPP inhibited STAT3 activity and expression of neuroinflammatory proteins, including ionized calcium binding adaptor molecule 1 (Iba1), inducible nitric oxide synthase (iNOS), and glial fibrillary acidic protein (GFAP) in 1-methyl-4-phenylpyridinium (MPP+; 0.5 mM)-treated primary cultured cells. However, mitogen-activated protein kinase (MAPK) inhibitors augmented the activity of MMPP. Collectively, our results suggest that MMPP may be an anti-inflammatory agent that attenuates dopaminergic neurodegeneration and neuroinflammation through MAO-B and MAPK pathway-dependent inhibition of STAT3 activation.
Keyphrases
- drinking water
- nitric oxide synthase
- cell proliferation
- high glucose
- oxidative stress
- anti inflammatory
- high performance liquid chromatography
- signaling pathway
- diabetic rats
- endothelial cells
- induced apoptosis
- drug induced
- binding protein
- nitric oxide
- spinal cord
- mass spectrometry
- stem cells
- pi k akt
- transcription factor
- poor prognosis
- lipopolysaccharide induced
- single cell
- health risk assessment
- immune response
- simultaneous determination
- health risk
- spinal cord injury
- mesenchymal stem cells
- tyrosine kinase
- nuclear factor
- solid phase extraction
- cerebral ischemia
- combination therapy
- toll like receptor
- cognitive impairment
- cell death
- high fat diet induced
- preterm birth