X-ray nano-tomography of complete scales from the ultra-white beetles Lepidiota stigma and Cyphochilus.
Stephanie L BurgAdam L WashingtonJulie VillanovaAndrew J C DennisonDaragh McLoughlinOleksandr O MykhaylykPete VukusicWill FurnassRichard A L JonesAndrew J ParnellJohn Patrick Anthony FaircloughPublished in: Scientific data (2020)
High resolution X-ray nano-tomography experiments are often limited to a few tens of micrometer size volumes due to detector size. It is possible, through the use of multiple overlapping tomography scans, to produce a large area scan which can encompass a sample in its entirety. Mounting and positioning regions to be scanned is highly challenging and normally requires focused ion beam approaches. In this work we have imaged intact beetle scale cells mounted on the tip of a needle using a micromanipulator stage. Here we show X-ray holotomography data for single ultra-white scales from the beetles Lepidiota stigma (L. stigma) and Cyphochilus which exhibit the most effective scattering of white light in the literature. The final thresholded matrices represent a scan area of 25 × 70 × 362.5 µm and 25 × 67.5 × 235µm while maintaining a pixel resolution of 25 nm. This tomographic approach allowed the internal structure of the scales to be captured completely intact and undistorted by the sectioning required for traditional microscopy techniques.
Keyphrases
- high resolution
- dual energy
- computed tomography
- electron microscopy
- mental health
- mental illness
- hiv aids
- social support
- mass spectrometry
- induced apoptosis
- systematic review
- tandem mass spectrometry
- image quality
- photodynamic therapy
- cell cycle arrest
- single molecule
- monte carlo
- magnetic resonance imaging
- depressive symptoms
- electronic health record
- ultrasound guided
- machine learning
- oxidative stress
- contrast enhanced
- antiretroviral therapy
- human immunodeficiency virus
- artificial intelligence
- endoplasmic reticulum stress
- high throughput
- single cell
- liquid chromatography