Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry.
Manami KobayashiJunichi MasudaNaomasa OshiroPublished in: Toxins (2024)
Ciguatera poisoning (CP) is the most common type of marine biotoxin food poisoning worldwide, and it is caused by ciguatoxins (CTXs), thermostable polyether toxins produced by dinoflagellate Gambierdiscus and Fukuyoa spp. It is typically caused by the consumption of large fish high on the food chain that have accumulated CTXs in their flesh. CTXs in trace amounts are found in natural samples, and they mainly induce neurotoxic effects in consumers at concentrations as low as 0.2 µg/kg. The U.S. Food and Drug Administration has established CTX maximum permitted levels of 0.01 µg/kg for CTX1B and 0.1 µg/kg for C-CTX1 based on toxicological data. More than 20 variants of the CTX1B and CTX3C series have been identified, and the simultaneous detection of trace amounts of CTX analogs has recently been required. Previously published works using LC-MS/MS achieved the safety levels by monitoring the sodium adduct ions of CTXs ([M+Na] + > [M+Na] + ). In this study, we optimized a highly sensitive method for the detection of CTXs using the sodium or lithium adducts, [M+Na] + or [M+Li] + , by adding alkali metals such as Na + or Li + to the mobile phase. This work demonstrates that CTXs can be successfully detected at the low concentrations recommended by the FDA with good chromatographic separation using LC-MS/MS. It also reports on the method's new analytical conditions and accuracy using [M+Li] + .
Keyphrases
- liquid chromatography
- tandem mass spectrometry
- klebsiella pneumoniae
- mass spectrometry
- ultra high performance liquid chromatography
- simultaneous determination
- high resolution mass spectrometry
- high performance liquid chromatography
- gas chromatography
- solid state
- label free
- loop mediated isothermal amplification
- human health
- real time pcr
- drug administration
- ion batteries
- escherichia coli
- quantum dots
- high resolution
- heavy metals
- randomized controlled trial
- risk assessment
- molecular docking
- drinking water
- health risk
- machine learning
- systematic review
- dna methylation
- molecularly imprinted
- data analysis