Transcription Cofactor CsMBF1c Enhances Heat Tolerance of Cucumber and Interacts with Heat-Related Proteins CsNFYA1 and CsDREB2.
Bingwei YuYonggui LiangQiteng QinYafei ZhaoChenyu YangRenjian LiuYuwei GanHuoyan ZhouZhengkun QiuLetian ChenShuangshuang YanBihao CaoPublished in: Journal of agricultural and food chemistry (2024)
Multiprotein bridging factor 1 (MBF1) is a very important transcription factor (TF) in plants, whose members influence numerous defense responses. Our study found that MBF1c in Cucurbitaceae was highly conserved. CsMBF1c expression was induced by temperature, salt stress, and abscisic acid (ABA) in cucumber. Overexpressed CsMBF1c enhanced the heat resistance of a cucumber, and the Csmbf1c mutant showed decreased resistance to high temperatures (HTs). CsMBF1c played an important role in stabilizing the photosynthetic system of cucumber under HT, and its expression was significantly associated with heat-related TFs and genes related to protein processing in the endoplasmic reticulum (ER). Protein interaction showed that CsMBF1c interacted with dehydration-responsive element binding protein 2 (CsDREB2) and nuclear factor Y A1 (CsNFYA1). Overexpression of CsNFYA1 in Arabidopsis improved the heat resistance. Transcriptional activation of CsNFYA1 was elevated by CsMBF1c. Therefore, CsMBF1c plays an important regulatory role in cucumber's resistance to high temperatures.
Keyphrases
- transcription factor
- binding protein
- heat stress
- endoplasmic reticulum
- nuclear factor
- poor prognosis
- dna binding
- genome wide identification
- toll like receptor
- gene expression
- oxidative stress
- cell proliferation
- heat shock
- genome wide
- long non coding rna
- protein protein
- amino acid
- stress induced
- drug induced
- estrogen receptor
- arabidopsis thaliana