Effect of up-regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma.
Zhanwang WangPeng WeiDongmin WeiShengda CaoHeng LiuLong ChenXiao HanXiaoyan ZhaoChuan LiuGuojun LiJianming YangXinliang PanDapeng LeiPublished in: Journal of cellular and molecular medicine (2020)
Increasing number of circular RNAs (circRNAs) have been reported to play important role in gene regulation, carcinogenesis and pathogenesis in various cancers. However, the biological functions and underlying molecular mechanisms of circRNAs in hypopharyngeal squamous cell carcinoma (HSCC) remain elusive. Thus, secondary circRNA-seq profiling was performed to identify the differentially expressed circRNAs between HSCC tissues and adjacent normal tissues, and the expression level of circMATR3 (derived from human gene matrin3 (MATR3), has_circRNA_0008922) was confirmed by qRT-PCR. Proliferation of HSCC cells was detected by cell counting kit-8 (CCK8) assay, apoptosis and the cell cycle were analysed by flow cytometry, and the migration and invasion of HSCC cells was determined by transwell assay. Bioinformatics analysis was conducted to predict possible pathways and potential miRNA targets of circMATR3. We found that circMATR3 was up-regulated in HSCC tissues, and abundant circMATR3 expression was markedly correlated with late T classification, advanced clinical stage, greater lymph node metastasis, and poor prognosis. Furthermore, knock-down of circMATR3 significantly inhibited proliferation, migration and invasion of HSCC cells, whereas silencing of circMATR3 induced cell apoptosis. Our analysis predicted that circMATR3 may participate in cancer-related pathways by serving as miRNA sponges. In conclusion, our findings first identified the oncogenic roles of circMATR3 in promoting the progression of HSCC and demonstrated that circMATR3 may be a novel prognostic marker and therapeutic target for HSCC.
Keyphrases
- poor prognosis
- cell cycle arrest
- induced apoptosis
- squamous cell carcinoma
- lymph node metastasis
- cell cycle
- signaling pathway
- long non coding rna
- endoplasmic reticulum stress
- cell death
- flow cytometry
- single cell
- cell proliferation
- gene expression
- pi k akt
- oxidative stress
- transcription factor
- high throughput
- endothelial cells
- machine learning
- high glucose
- young adults
- rna seq
- cell therapy
- diabetic rats
- stress induced
- genome wide analysis