Nanopore sequencing and full genome de novo assembly of human cytomegalovirus TB40/E reveals clonal diversity and structural variations.
Timokratis KaramitrosBonnie van WilgenburgMark WillsPaul KlenermanGkikas MagiorkinisPublished in: BMC genomics (2018)
Third generation high-throughput sequencing technologies can accurately reconstruct full-length HCMV genomes including their low-complexity and highly repetitive regions. Full-length HCMV genomes could prove crucial in understanding the genetic determinants and viral evolution underpinning drug resistance, virulence and pathogenesis.
Keyphrases
- high throughput sequencing
- endothelial cells
- genome wide
- escherichia coli
- pseudomonas aeruginosa
- staphylococcus aureus
- high frequency
- sars cov
- mycobacterium tuberculosis
- epstein barr virus
- single cell
- single molecule
- antimicrobial resistance
- biofilm formation
- pluripotent stem cells
- copy number
- gene expression
- dna methylation
- diffuse large b cell lymphoma