Validation of SYBR green I based closed tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL).

Keerti Kaumudee DixitSandeep VermaOm Prakash SinghDharmendra SinghAkhil Pratap SinghRatan GuptaNarendra Singh NegiPradeep DasShyam SundarRuchi SinghPoonam Salotra

Published in: PLoS neglected tropical diseases (2018)

The validated closed tube LAMP for diagnosis of VL will provide impetus to the ongoing VL elimination programme in ISC. The assay based on direct blood lysis promotes its scope for application in field settings by further reducing time and cost.

Keyphrases

loop mediated isothermal amplification
sensitive detection
high throughput
study protocol
clinical trial
randomized controlled trial