Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme.

Yonghao ZhanCongcong CaoAolin LiHongbing MeiYuchen Liu

Published in: Genome biology (2023)

Thus, our study demonstrates that CCRS expands the design ideas and RNA knockdown capabilities of Cas13 technology and has the potential to be used in disease treatment.

Keyphrases

crispr cas
genome editing
genome wide
nucleic acid
gene expression
risk assessment
replacement therapy