A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 1⁻5 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL.
Keyphrases
- multidrug resistant
- drug resistant
- klebsiella pneumoniae
- escherichia coli
- poor prognosis
- acinetobacter baumannii
- staphylococcus aureus
- high resolution
- magnetic resonance
- binding protein
- molecular docking
- biofilm formation
- crispr cas
- saccharomyces cerevisiae
- cystic fibrosis
- candida albicans
- clinical evaluation
- wound healing