The Prophylactic Use of Bovine Colostrum in a Murine Model of TNBS-Induced Colitis.
Menchetti LauraGiulio CuroneIulia Elena FilipescuOlimpia BarbatoLeonardo LeonardiGabriella GuelfiGiovanna TrainaPatrizia Casagrande-ProiettiFederica RivaAnna Beatrice CasanoFederica PiroDaniele VigoAlda QuattroneGabriele BrecchiaPublished in: Animals : an open access journal from MDPI (2020)
This study investigated the effects of a short-term administration of bovine colostrum (BC) in a TNBS model of induced colitis. Colitis was induced by TNBS treatment after seven days of BC (BC group, n = 12) or saline (control group, n = 12) administration in mice. Clinical signs, histopathological characteristics, expression levels of Toll-like receptor 4 (TLR4), pro- and anti-inflammatory cytokines, and microbial composition were assessed. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, the BC group showed a reduction in body weight (BW) loss compared to Control (p < 0.05). Moreover, expression levels of TLR4 (p < 0.01), Interleukin-1β (IL-1β; p < 0.001), Interleukin-8 (IL-8; p < 0.001), and Interleukin-10 (IL-10; p < 0.001) were lower in mice administered with BC. Finally, Escherichia coli were higher (p < 0.05), while Enterococci (p < 0.001), Lactobacillus spp. (p < 0.001), and Bifidobacterium spp. (p < 0.05) were lower in Control than BC group. This study confirms that pre-treatment with BC modulates the expression of genes and the count of microbes involved in the etiopathogenesis of colitis.
Keyphrases
- toll like receptor
- poor prognosis
- escherichia coli
- inflammatory response
- body weight
- immune response
- nuclear factor
- type diabetes
- long non coding rna
- microbial community
- binding protein
- pseudomonas aeruginosa
- gene expression
- oxidative stress
- adipose tissue
- genome wide
- dna methylation
- high resolution
- cystic fibrosis
- preterm infants
- insulin resistance
- mass spectrometry
- physical activity
- smoking cessation
- biofilm formation
- high speed
- candida albicans
- atomic force microscopy