Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model.
Yoon Jin RohYong Hee ChoiSun Hye ShinMi-Kyung LeeYu Jin WonJun Ho LeeByong Seung ChoKui Young ParkSeong Jun SeoPublished in: PloS one (2024)
Recently, particulate matter (PM) has been shown to exacerbate atopic dermatitis (AD) by inducing an inflammatory response. Meanwhile, several studies revealed that exosomes derived from adipose tissue-derived mesenchymal stem cells promote wound healing and alleviate inflammation via their regenerative and immunomodulatory capacities. Our study aimed to investigate the effects of human adipose tissue-derived mesenchymal stem cell-derived (ASC)-exosomes in PM-induced AD. An AD-like triple-cell model was established by treating human keratinocytes, dermal fibroblasts, and mast cells with polyinosinic:polycytidylic acid (Poly I:C) and interleukin 1 alpha (IL-1α). The effects of PM and ASC-exosomes on the expression of pro-inflammatory cytokines and skin barrier proteins were examined using quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence. PM increased pro-inflammatory cytokines (IL-6, IL-1β, and IL-1α) and decreased the anti-inflammatory cytokine IL-10, while the mRNA expression of skin barrier proteins (loricrin and filaggrin) decreased. However, when the cells were treated with ASC-exosomes, the PM-induced effects on pro-inflammatory cytokines and skin barrier proteins were reversed. Our results confirmed that PM-induced inflammation and skin barrier damage were alleviated by ASC-exosomes in our AD-like triple-cell model. These data suggest that ASC-exosomes can serve as a therapeutic agent for PM-exacerbated AD.
Keyphrases
- particulate matter
- air pollution
- mesenchymal stem cells
- wound healing
- stem cells
- adipose tissue
- atopic dermatitis
- inflammatory response
- high glucose
- anti inflammatory
- oxidative stress
- cell therapy
- diabetic rats
- endothelial cells
- single cell
- soft tissue
- nlrp inflammasome
- drug induced
- poor prognosis
- high fat diet
- induced apoptosis
- heavy metals
- risk assessment
- immune response
- machine learning
- bone marrow
- lps induced
- lipopolysaccharide induced
- metabolic syndrome
- artificial intelligence