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Porcine germline genome engineering.

Luhan YangGeorge M ChurchHong-Ye ZhaoLusheng HuangYangbin GaoHong-Jiang WeiGeoffrey Yang
Published in: Proceedings of the National Academy of Sciences of the United States of America (2021)
Germline editing, the process by which the genome of an individual is edited in such a way that the change is heritable, has been applied to a wide variety of animals [D. A. Sorrell, A. F. Kolb, Biotechnol. Adv. 23, 431-469 (2005); D. Baltimore et al., Science 348, 36-38 (2015)]. Because of its relevancy in agricultural and biomedical research, the pig genome has been extensively modified using a multitude of technologies [K. Lee, K. Farrell, K. Uh, Reprod. Fertil. Dev. 32, 40-49 (2019); C. Proudfoot, S. Lillico, C. Tait-Burkard, Anim. Front. 9, 6-12 (2019)]. In this perspective, we will focus on using pigs as the model system to review the current methodologies, applications, and challenges of mammalian germline genome editing. We will also discuss the broad implications of animal germline editing and its clinical potential.
Keyphrases
  • crispr cas
  • genome editing
  • dna repair
  • genome wide
  • public health
  • risk assessment
  • dna damage
  • oxidative stress
  • dna methylation