Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.

Gabriel LevinBruna Andrade Aguiar KogaGustavo Gross BelchiorAna Claudia Oliveira CarreiraMari Cleide Sogayar

Published in: BMC biotechnology (2020)

Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.

Keyphrases

recombinant human
induced apoptosis
tissue engineering
cell cycle arrest
cell death
protein protein
oxidative stress
amino acid
single cell
cell free
pluripotent stem cells