Cell type transcriptome atlas for the planarian Schmidtea mediterranea.
Christopher T FincherOmri WurtzelThom de HoogKellie M KravarikPeter W ReddienPublished in: Science (New York, N.Y.) (2018)
The transcriptome of a cell dictates its unique cell type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal: the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. We generated data for 66,783 cells, defining transcriptomes for known and many previously unknown planarian cell types and for putative transition states between stem and differentiated cells. We also uncovered regionally expressed genes in muscle, which harbors positional information. Identifying the transcriptomes for potentially all cell types for many organisms should be readily attainable and represents a powerful approach to metazoan biology.
Keyphrases
- single cell
- rna seq
- high throughput
- induced apoptosis
- cell cycle arrest
- cell therapy
- stem cells
- mesenchymal stem cells
- dna methylation
- machine learning
- cell death
- gene expression
- endoplasmic reticulum stress
- signaling pathway
- skeletal muscle
- oxidative stress
- health information
- multidrug resistant
- data analysis
- big data
- pluripotent stem cells