Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe.
Faithful Makita-ChingombeAnthony T PodanyTimothy M MykrisFarai MuzambiRichard W BrowneAndrew J OcqueRobin DiFrancescoLee C WinchesterCourtney V FletcherTinashe MudzvitiCharles C MapongaGene D MorsePublished in: African journal of laboratory medicine (2021)
An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.
Keyphrases
- high performance liquid chromatography
- hiv testing
- tandem mass spectrometry
- mass spectrometry
- solid phase extraction
- simultaneous determination
- men who have sex with men
- antiretroviral therapy
- clinical trial
- hiv positive
- liquid chromatography
- human immunodeficiency virus
- hiv infected
- ms ms
- randomized controlled trial
- high throughput
- loop mediated isothermal amplification
- study protocol
- label free
- south africa
- sensitive detection