Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq.

Michele BusbyCatherine XueCatherine LiYossi FarjounElizabeth GiengerIdo YofeAdrianne GladdenCharles B EpsteinEvan M CornettScott B RothbartChad NusbaumAlon Goren

Published in: Epigenetics & chromatin (2016)

Altogether, we found that monoclonal antibodies as a class perform equivalently to polyclonal antibodies for the detection of histone post-translational modifications in both human and mouse. Accordingly, we recommend the use of monoclonal antibodies in ChIP-seq experiments.

Keyphrases

genome wide
dna methylation
single cell
high throughput
rna seq
circulating tumor cells
endothelial cells
high resolution
pluripotent stem cells
multiple myeloma
high density
label free