Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies.

Kamila Gaudêncio da Silva SalesDébora Elienai de Oliveira MirandaMarcelo Henrique Santos PaivaLuciana Aguiar FigueredoDomenico OtrantoFilipe Dantas-Torres

Published in: Parasites & vectors (2020)

This multiplex real-time PCR assay represents a novel fast assay for detecting dog, human and Leishmania DNA in female sand flies and therefore a tool for assessing the risk of Leishmania transmission to these hosts in areas of active transmission.

Keyphrases

real time pcr
endothelial cells
high throughput
drinking water
induced pluripotent stem cells
pluripotent stem cells
single molecule
cell free
single cell
plasmodium falciparum
sensitive detection