MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium.
Saemee SongBoeun LeeJi-Hyun YeomSoonhye HwangIlnam KangJang-Cheon ChoNam-Chul HaJeehyeon BaeKangseok LeeYong-Hak KimPublished in: Infection and immunity (2015)
MdsABC is a Salmonella-specific tripartite efflux pump that has been implicated in the virulence of Salmonella enterica serovar Typhimurium; however, little is known about the virulence factors associated with this pump. We observed MdsABC expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. Thin-layer chromatography and tandem mass spectrometry analyses revealed that overexpression of MdsABC led to increased secretion of 1-palmitoyl-2-stearoyl-phosphatidylserine (PSPS), affecting the ability of the bacteria to invade and survive in host cells. Overexpression of MdsABC and external addition of PSPS similarly rendered the mdsABC deletion strain resistant to diamide. Diagonal gel analysis showed that PSPS treatment reduced the diamide-mediated formation of disulfide bonds, particularly in the membrane fraction of the bacteria. Salmonella infection of macrophages induced the upregulation of MdsABC expression and led to an increase of intracellular bacterial number and host cell death, similar to the effects of MdsABC overexpression and PSPS pretreatment on the mdsABC deletion strain. Our study shows that MdsABC mediates a previously uncharacterized pathway that involves PSPS as a key factor for the survival and virulence of S. Typhimurium in phagocytic cells.
Keyphrases
- listeria monocytogenes
- escherichia coli
- tandem mass spectrometry
- induced apoptosis
- cell cycle arrest
- poor prognosis
- cell death
- cell proliferation
- biofilm formation
- pseudomonas aeruginosa
- oxidative stress
- staphylococcus aureus
- ultra high performance liquid chromatography
- high performance liquid chromatography
- liquid chromatography
- antimicrobial resistance
- signaling pathway
- diabetic rats
- endoplasmic reticulum stress
- mass spectrometry
- simultaneous determination
- gas chromatography
- adipose tissue
- high resolution
- pi k akt
- long non coding rna
- high resolution mass spectrometry
- ischemia reperfusion injury
- high speed
- combination therapy
- ms ms
- reactive oxygen species
- hyaluronic acid
- heat stress