An LCMS-based untargeted metabolomics protocol for cochlear perilymph: highlighting metabolic effects of hydrogen gas on the inner ear of noise exposed Guinea pigs.

Kristian PirttiläPernilla Videhult PierreJakob HaglöfMikael EngskogMikael HedelandGöran LaurellTorbjörn ArvidssonCurt Pettersson

Published in: Metabolomics : Official journal of the Metabolomic Society (2019)

A HILIC-UHPLC-Q-TOF-MS-based protocol for untargeted metabolomics of perilymph is presented and shown to be fit-for-purpose. We found a clear difference in the perilymph metabolome of noise exposed guinea pigs with and without H2 treatment.

Keyphrases

mass spectrometry
liquid chromatography
high resolution mass spectrometry
randomized controlled trial
air pollution
ms ms
gas chromatography
high resolution
simultaneous determination
ultra high performance liquid chromatography
carbon dioxide