Diterpenoids from Plectranthus spp. as Potential Chemotherapeutic Agents via Apoptosis.
Tomasz ŚliwińskiPrzemysław SitarekEwa SkałaVera M S IscaEwelina SynowiecTomasz KowalczykMichał BijakPatrícia RijoPublished in: Pharmaceuticals (Basel, Switzerland) (2020)
Plectranthus spp. is widely known for its medicinal properties and bioactive metabolites.The cytotoxic and genotoxic properties of the four known abietane diterpenoids: 7α-Acetoxy-6β-hydroxyroyleanone (Roy), 6,7-dehydroroyleanone (Deroy), 7β,6β-dihydroxyroyleanone6 (Diroy),and Parvifloron D (Parv), isolated from P. madagascariensis (Roy, DeRoy, and Diroy) and P. ecklonii(Parv) were evaluated. The tested compounds showed cytotoxic effects against the human leukemiacell line CCRF-CEM and the lung adenocarcinoma cell line A549. All tested compounds inducedapoptosis by altering the level of pro- and anti-apoptotic genes. The results show that from the testedditerpenoids, Roy and Parv demonstrated the strongest activity in both human cancer cell lines,changing the permeability mitochondrial membrane potential and reactive oxygen species (ROS)levels, and possibly inducing mtDNA or nDNA damage. In conclusion, the abietane diterpenoidstested may be used in the future as potential natural chemotherapeutic agents.
Keyphrases
- endothelial cells
- reactive oxygen species
- oxidative stress
- cell death
- induced pluripotent stem cells
- human health
- dna damage
- pluripotent stem cells
- papillary thyroid
- cell cycle arrest
- squamous cell carcinoma
- risk assessment
- current status
- dna methylation
- squamous cell
- lymph node metastasis
- genome wide identification